determination of lethal dose of plant extract

Anthelmintic efﬁcacy and dose determination of Albizia

Anthelmintic efﬁcacy and dose determination of Albizia anthelmintica against gastrointestinal nematodes in naturally infected Ugandan sheep J.T. Gradea,b,*, B.L. Arblec, R.B. Weladjid, P. Van Dammea aLaboratory of Tropical and Subtropical Agronomy and Ethnobotany, Faculty of Bioscience Engineering, 653 Coupure links, Ghent University, B-9000 Gent, Belgium Get price

DETERMINATION OF MEDIAN LETHAL DOSE OF ETHANOLIC

of the extract considering it`s lipid nature. The concentration obtained was 12,830mg/ml which is equal to 13.297ml when measured using a measuring cylinder. The equivalent dose of 10mg in ml was measured and the value 0.0104ml was obtained. Similar measurement was made for other doses and the values (0.104, 1.04, 1.66, Get price

Toxicity Evaluation of the Extract and Fraction of

extract and butanol fraction of C. albidum seed cotyledons following acute and 28-day repeated dosing was investigated in rats using OECD Test Guidelines 420 and 407. The median lethal dose (LD50) of the extract and fraction were 760 and 200 mg/kg respectively, and both effect varying degree of significant changes at p 0.05 in Get price

Phytochemical Screening and Acute Oral Toxicity Study of

Thus, the possible oral lethal dose for microwave dried Java tea leaves is more than 5000 mg/Kg body weight. 1. Introduction. Nowadays, the usage of products of natural origin such as those derived from plant, animal, or marine sources as health supplements, revitalizers, and agents in Get price

Phytochemical Screening and Antimicrobial Activities of

Zaika LL. Spices and herbs: their antimicrobial activity and its determination. J Food Saf. 1988; 9(2): 97-118, CrossRef. Akinyemi KO, Oluwa OK, Omomigbehin EO. Antimicrobial activity of crude extracts of three medicinal plants used in south-west Nigerian folk Get price

The Suicide Methods file V2.0 (unverified), natural poisons

The lethal dose for a horse is 3-5 kg fresh plants; for pigs, it is 8-13 grams per kilo bodyweight; for cows, 5.3 gram of fresh plants per kilo bodyweight (i.e.16 mg coniin per kilo bodyweight); for sheep, 10 gram fresh plants per kilo bodyweight (or 240 mg coniin per kilo Get price

In vivo antiplasmodial activity and toxicological

Jan 13, 2017Accordingly, 2000 mg/kg of each plant extract was found safe, i.e., the approximate median lethal dose (LD 50) of the extracts in the experimental mice was higher than 2000 mg/kg. The results observed in the acute toxicity study with A. remota is in Get price

determination of median lethal dose of triazophos

Vol 7, Issue 4, 2014 ISSN - 0974-2441 Research Article DETERMINATION OF MEDIAN LETHAL DOSE OF TRIAZOPHOS WITH DMSO IN WISTAR RATS MOHINEESH CHANDRA1, JAYA RAJ1, TIRATH DAS DOGRA2, AVINASH CHANDER RAJVANSHI3, ANUPUMA RAINA1 Department of Forensic Medicine and Toxicology, All India Institute of Medical Sciences, New Delhi, India. 2Vice Chancellor, Get price

Review of the Safety and Efficacy of Moringa oleifera

Mar 24, 2015determined that the lethal dose of 50% of an orally administered ethanol extract of M. oleifera leaves in mice was greater than 6.4 g/kg. The dietary effects of M. oleifera leaves as a dietary supplement for liver function were performed by Zvinorova et al . Get price

In vitro cytotoxic activity of medicinal plants from

Nov 22, 2017Brine shrimp lethality assay. The result obtained showed that the activity of all the plant extracts were concentration-dependent. All the plant extracts, with the exception of Nauclea diderrichii extract, had LC 50 value below 1000 μg/mL indicating the presence of bioactive secondary metabolites in these plants. Eleusine indica extract (LC 50 = 76.3 μg/mL) had the highest cytotoxicity on Get price

Evaluation of Acute Toxicity of the Methanolic Extract of

Safety of polyherbal extract is evaluated principally by estimation of acute oral toxicity. Within the study, even the highest dose of plant extract i.e. 4000 mg/kg did not show any symptoms of toxication and death in for animals. Therefore, this polyherbal extract even at 4000 mg/kg could also be recommended as safe and offers no harm to the Get price

Antibacterial and Ftir Spectral Analysis of Methanolic

Plants extracts contain 2.5 Quantitative Determination of the Lethal Effect of the Extract on the Bacteria Isolates Firstly to carry out lethal dose test of the extract, the density of bacterial suspension inoculated onto the media for lethal test was determined by comparison with 0.5 McFarland standard of Get price

Assessment of Antioxidant Potential and Acute Toxicity

Objective: The aim of this study is to evaluate the in vitroantioxidant potential and to determine the median lethal dose (LD50) of crude ethyl acetate and methanol extracts of Pergularia daemia. The plant Pergularia daemiapossess effective scavenging activity against 2, 2' azino bis (3 ethylbenzothiazoline 6 sulfonic acid (ABTS), nitric oxide and reducing power radicals at different Get price

Antiulcerogenic activity of Solenostemon monostachyus

The phytochemical screening of the ethanolic extract of the whole plant of Solenostemon monostachyus revealed the presence of alkaloids, cardiac glycosides, tannins, saponins, terpenes and flavonoids. Determination of median lethal dose (LD 50) The median lethal dose (LD Get price

Acute toxicity of Brosimum gaudichaudii Trcul. root

The 2000 mg/kg dose is established as a dose ceiling of the plant extract for the determination of DL50 because, above this value, the result obtained may be considered out of the product's standards for therapeutic use and, therefore, unrealistic, without significance under the conditions foreseen for the its use (Brasil, 1996; WHO, 1993; Lapa Get price

STUDY THE PATHOLOGICAL EFFECTS OF CRUDE EXTRACT OF

2- Median lethal dose Graduated doses of Portulaca oleracea ethanolic extract were dissolved in 10 ml distill water and administered S/C as 0.1 ml for each 10 gm of animal body weight. The range was of S/C single doses used in the determination of LD50 of the extract was (5000- 9500) mg /kg B.W. Get price

Introduction to toxicology

3 Toxicology Philippus Theophrastus Aureolus Bombastus von Hohenheim PARACELSUS (Einsiedeln, Zrich, 1493 - Salzburg, 1541) All substances are poisons; it is the dose that makes the poison The historical development of toxicology began with early cave dwellers who recognized poisonous plants and animals and used their extracts for hunting or in warfare. Get price

International Journal of Current Microbiology and Applied

Determination of percentage yield The percentage yield of the extract was calculated using the following formular % yield = Assay of biological activity Acute toxicity and lethality Investigation on acute toxicity of the extract with estimation of the median lethal dose (LD 50) Get price

Vom Journal of Veterinary Science Vol. 9, 2012: 25 31

terpenoids, flavonoids and alkaloid. The median lethal dose of the plant was greater than 5000 mg/kg body weight. The ethanolic extract did not cause death or signs of toxicity in the rats but significantly (p0.05, p0.01) decreased blood glucose. In conclusion, Telfairia Get price

Evaluation Of Hypoglycemic Potential Of Extracts Of

investigate acute toxicity of the plant extract on wistar albino rats. Lethal Median Dose (LD 50) was calculated using the formula below: LD 50 = (the apparent least dose lethal to animals – [(a x b)/N]) Where, N = number of animal used, a = dose difference, and b = mean mortality. NB: For the study, 1/10th of LD 50 was used. Get price

Review of the Safety and Efficacy of Moringa oleifera

Mar 24, 2015determined that the lethal dose of 50% of an orally administered ethanol extract of M. oleifera leaves in mice was greater than 6.4 g/kg. The dietary effects of M. oleifera leaves as a dietary supplement for liver function were performed by Zvinorova et al . Get price

22.4: The Lethal Dose

Aug 10, 2020The LD 50 is a standardized measure for expressing and comparing the toxicity of chemicals. The LD 50 is the dose that kills half (50%) of the animals tested (LD = lethal dose). The animals are usually rats or mice, although rabbits, guinea pigs, hamsters, and so on are sometimes used. In all these tests, the dose must be calculated relative to the size of the animal. Get price

EVALUATION OF THE LETHAL DOSE OF THE METHANOL

indiscriminate use of plant medicine without prejudice to the toxic effects of plant extracts of which the leaf of mangrove is one, the present investigation aims to determine the mean lethal dose (LD 50) of leaf extract of Rhizophora racemosa collected from Dodo River in Bayelsa State, Nigeria. MATERIALS AND METHODS Get price

Chapter 5: ACUTE TOXICITY DEFINITIONS CLASSIFICATION

11. The criteria for substances classify acute toxicity by use of lethal dose data (tested or derived). For mixtures, it is necessary to obtain or derive information that allows the criteria to be applied to the mixture for the purpose of classification. The approach to classification for acute Get price

Evaluation of the toxicity and antiulcerogenic activity of

Parra AL, Yhebra RS, Sardias IG, Buela LI 2001. Comparative study of the assay of Artemia salina L. and the estimate of the medium lethal dose (LD 50 value) in mice, to determine oral acute toxicity of plant extracts. Phytomedicine 8: 395-400. [ Links ] Get price